A Case of Intravascular Lymphoma with Increased Regional Cerebral Blood Flow in I-123 IMP Single-Photon Emission CT

Chihoko Miyazaki; Masaya Mukai; Yuriko Kawaai; Miki Takeda; Norio Katoh; Syunsuke Nagano; Kozoh Kubo; Michifumi Kohno

Abstract

Summary: This report documents a case of intravascular lymphoma (IVL) with increased regional cerebral blood flow (rCBF) disclosed at I-123 IMP single-photon emission CT (IMP-SPECT). A 73-year-old woman with IVL had high rCBF disclosed by IMP-SPECT before chemotherapy; rCBF was normal after one course of CHOP (cyclophophamide, vincristine, doxorubicin, and prednisone) chemotherapy. During her clinical course, she had an episode in which she showed increased rCBF in the left cerebral hemisphere at IMP-SPECT performed immediately after the recovery from the right hemiparesis.

Intravascular lymphoma (IVL) is a rare, generally fatal, disease characterized by multifocal proliferation of lymphoma cells within the lumen of small vessels throughout the body. CNS involvement usually presents as subacute encephalopathy, dementia, seizure, or multifocal cerebrovascular events. Recently, CT and MR findings of the brain as multiple patchy white matter lesions or infarct-like lesions in IVL have been reported. By contrast, it has been known that IVL clinically mimics primary angiitis of CNS, and cerebral angiography reveals findings consistent with those of CNS vasculitis. This case may support that CNS manifestations of early-stage IVL mimic CNS vasculitis or encephalitis.

Case Report

A 73-year-old woman who was suspected of having malignant lymphoma after several weeks’ history of general fatigue, anorexia, high fever, pancytopenia, high levels of lactate dehydrogenase (LDH) and C-reactive protein (CRP), and phagocytosis in the bone marrow was admitted to our hospital in late November 1999. Laboratory findings on admission showed the following values: erythrocyte sedimentation rate, 62 mm/h; leukocytes, 5000/μL; erythrocytes, 266 × 10⁴/μL; hemoglobin, 8.9 g/dL; hematocrit, 25.3%; platelets, 3.9 × 10⁴/μL; CRP, 9.45 mg/dL; LDH, 3359 IU/L; soluble interleukin-2 receptor (sIL-2R), 13044u/mL. Bone marrow biopsy revealed diffuse large cell lymphoma that was positive for the leukocyte common antigen (LCA), and the B-cell marker. CSF protein level was 48 mg/dL. The cell number of CSF was 19/3, and cytology was class III of lymphocytes with enlarged nuclei. On the basis of clinical data and symptoms, intravascular lymphoma was diagnosed.

Brain MR imaging findings (Fig 1) showed minimal high intensity in the basal ganglia (BG) and thalamus (Th) on T2-weighted images, which may suggest minimal lacunar infarcts. A contrast-enhanced T1-weighted coronal image showed dural-arachnoid enhancement. On contrast-enhanced T1-weighted images, the bone marrow of the skull showed signal intensity prolongation, which implies bone marrow invasion or changes to red marrow due to anemia. On CT examination, no lymph node enlargement was noted anywhere in the chest or the abdomen. The other abnormal CT findings were splenomegaly with several low-attenuation areas and small amounts of ascites and pleural effusions.

Fig 1.

Images were obtained at the initial admission before chemotherapy began. Axial T2-weighted (3500/90) MR images (A) reveal minimum high-signal-intensity lesions in the both thalamus and putamen bilaterally. Coronal T1-weighted (458/14) MR image (B) shows that relatively thick meninges and bone marrow of the skull are enhanced after contrast material administration.

IMP single-photon emission computed tomography (IMP-SPECT) (Fig 2) studies were performed by using a three-head rotating gamma camera (GCA-9300A/DI, Tokyo, Japan) with a low-energy, fan-beam, high-resolution collimator. Data were obtained by continuous rotation in a 128 × 128 matrix. The patient was blindfolded for 10 minutes before and after the injection. Data collection for SPECT images was initiated 20 minutes after the intravenous administration of 222 MBq I-123 IMP, which lasted for 1 minute. A filtered back-projection technique with Butterworth (cutoff frequency 0.10 cycles/pixel; order 8) and ramp filters were applied for image reconstruction. All data were collected at an attenuation of 0.146/cm by using the method of Chang. Image acquisition time was 20 minutes, and each section was 6.9 mm thick. Image sections were set up parallel to the orbitomeatal line. Regional CBF measurement was performed by obtaining single arterial blood sampling from the brachial artery 10 minutes after IMP infusion on the basis of the ARG method (1). The distribution volume was fixed (V_d = 45 mL/mL), and the standard input function was referred to the data of Research Institute for Brain and Blood Vessels, in Akita, Japan. Three axial images through the midsection of the cerebellum and the Th and the BG and just above the lateral ventricles were selected to create irregular regions of interests for the measurements of rCBF in the cerebral cortical areas of each territory under anterior, middle, and posterior cerebral arteries, BG, Th, semioval center, cerebellum, and brain stem.

Fig 2.

rCBF images at three different sections calculated by the I-123 IMP ARG method before the first course of chemotherapy (A), after the first course of chemotherapy (B), 2 hours after the recovery from the left hemiparesis (C), and 2 months before death (D) are shown. The same color scale is used to display quantitative rCBF images through four sequential I-123 IMP SPECT studies.

Initial IMP-SPECT on admission showed very high rCBF throughout the brain (Fig 2A). The values of rCBF in each region of interest were summarized in sequential studies (Table 1). After two courses of half-dose combination chemotherapy of CHOP (cyclophophamide, vincristine, doxorubicin, and prednisone), rCBF in IMP-SPECT (Fig 2B) decreased to a level within normal range. In late March 2000, after a total of six courses of combination chemotherapy, the patient left the hospital. On discharge, sIL-2R was reduced to a level of 100s, and the bone marrow biopsy showed myeloid hyperplasia without lymphoma. Five months later, sIL-2R was increased again to 1888 IU/mL, and bone marrow biopsy showed the presence of lymphoma cells. After THP-COP (pirarubicin, cyclophophamide, vindesine, and prednisone) chemotherapy was carried out from September to November 2000, she received etoposide as an outpatient. On January 3, 2001, she was admitted for anorexia and progressive lethargy. Altered consciousness and mental status and progression of dementia were observed despite continuous THP-COP chemotherapy. On the morning of February 6, the day after chemotherapy, she was found to be right hemiparetic. Contrast-enhanced CT 6 hours after onset did not show any abnormal attenuation, except for increased vascular attenuation in the left cerebral hemisphere (Fig 3). On February 8, the patient demonstrated difficulty in speech. On February 9, approximately 2 hours after these symptoms disappeared and confusion was improved, IMP-PECT (Fig 2C) revealed high rCBF in the left cerebral hemisphere and the right cerebellum. On February 20, IMP-SPECT (Fig 2D) showed relatively high rCBF at similar degree in both the right and the left cerebral hemispheres. Nevertheless, several courses of chemotherapy could not suppress the progression of dementia, and the patient’s condition continued to worsen. She died 17 months after her initial admission. The summary of sequential examination findings and chemotherapy protocol is shown in Table 2.

Fig 3.

Images obtained on February 6, 2001, 10 hours after the onset of right hemiparesis. Postcontrast head CT shows that the vascular structures in the left cerebrum are enhanced as compared with those in the other side through the level of the third ventricle (A) and the body of the lateral ventricles (B).

View this table:

TABLE 1:

Summary of the values of rCBF calculated by the I-123 IMP ARG method in sequential studies

View this table:

TABLE 2:

Summary of sequential examination findings and chemotherapy protocol

Autopsy revealed that vascular structures in brain parenchyma were prominent, and sporadic lesions with dark color in the cortex of the parietal area were suggestive of infarcts. Microscopic examination showed lymphoma infiltration in meninges, subarachnoid space, Virchow-Robin space of parenchyma, cerebral and cerebellar parenchyma, pituitary gland, lumina, and wall of the small vessels of the brain parenchyma (Fig 4). Lesions with dark color were gliosis and demyelinated, which are findings that are compatible with infarct. B-cell lymphoma was also identified in the blood vessels of the spleen, lungs, liver, kidneys, adrenal glands, and parametrium.

Fig 4.

Histologic specimens (Hematoxylin-eosin stain) reveal infiltration of neoplastic lymphoid cells within the lumen of a small vessel of the brain parenchyma (A, ×400), especially concentrated in the Virchow-Robin perivascular space (B, ×200), and in the brain parenchyma (C, ×200).

Discussion

Intravascular lymphoma (IVL)—also known as malignant angioendotheliomatosis, angiotropic large-cell lymphoma, or intravascular lymphomatosis (2, 3)—was first described in 1959 as “angioendotheliomatosis proliferans systemisata” (4). It is a rare, generally fatal, disease characterized by a multifocal proliferation of an aggressive type of non- Hodgkin lymphoma cells within the lumen of small blood vessels and commonly affects the CNS and the skin (2, 3). According to the literature, IVL has a predilection for patients in their 6th to 7th decades of life, with a male-female ratio of 1: 2—that is, the incidence in men is twice that in women. The most common manifestations are fever of unknown origin or intermittent fevers, skin rash, and changes of mental status. CNS involvement usually manifests an encephalopathy ranging from acute disorientation to rapidly progressive dementia and focal signs such as hemiparesis and myelopathy (5–7). Although any organ may be involved, those typically involved in lymphoma, such as the lymph nodes and bone marrow, are rarely enlarged or expanded by the neoplastic process (8). The involvement of hematopoietic organs is an uncommon feature, and bone marrow examinations in patients with IVL are usually nondiagnostic (5, 9). In addition, CSF analysis may show mild leukocytosis or an increased amount of protein, but cytologic results are negative for either finding in most patients (10). In the case presented here, however, not only was lymphoma infiltration found in the bone marrow and CSF, but also a CT scan of the abdomen suggested lymphoma involvement of the spleen on the initial admission. On laboratory examination, this case demonstrated, in addition to increased sIL-2R, all of nonspecific laboratory abnormalities such as anemia, increased CRP and serum LDH levels, and elevated erythrocyte sedimentation rate as described in most reports.

Recently, CT and MR findings of the brain have been reported in IVL. Williams et al (11) retrospectively reviewed MR imaging data in four cases of IVL. These MR findings included infarct-like lesions (n = 2), focal parenchymal enhancement (n = 3), dural-arachnoid enhancement (n = 2), and in one case, nonspecific, patchy foci of increased signal intensity in the white matter on long-TR images. All patients had multifocal lesions. The MR findings arose in part from vessels occluded by IVL, producing a wide spectrum of abnormalities that might range from multifocal nonspecific white matter changes to infarct-like lesions and that the MR appearance of IVL might also manifest as enhancing mass lesions, possibly predicting extra luminal spread of disease. Terae et al (12) summarized the latest MR findings of 33 patients with IVL as follows: 1) multiple hyperintense lesions on T2-weighted images or fluid-attenuated inversion recovery images mainly in the cerebral white matter; 2) infarct-like lesions involving the cortical and subcortical regions; 3) lacunar infarct-like lesions in the basal ganglia, thalami, and brain stem; 4) diffuse hyperintense lesions in the cerebral white matter on T2-weighted images; 5) contrast enhancement varies from no enhancement to variable parenchymal enhancement, including either linear and punctuate, patchy, irregular, nodular, cortical, or periventricular enhancement, and meningeal enhancement such as dual, arachnoids, or pial enhancement; and 6) serial MR imaging might show development of new lesions or progression of existing lesions, fluctuation in size or number of the lesions in a relatively short period during corticosteroid therapy, or reduction of the lesion size after CHOP therapy. In the present case, however, initial MR imaging was essentially normal except for dural-arachnoid and postcontrast bone marrow enhancement at the time of the first admission. The reason there was no enhancement in the brain parenchyma with the initial MR imaging was mainly because the disease was at an early stage, and vessel occlusion had not occurred. The propensity for tumors to proliferate and remain within vascular spaces might have been minimal, whereas tumor infiltration in the wall of the small vessels and perivascular space of the brain parenchyma might have been slightly dominant; however, meninx and postcontrast bone marrow enhancement meant lymphoma infiltration. As the disease progresses, MR imaging must show the same cortical and white matter lesions as identified at autopsy.

Diffusion-weighted images, perfusion MR imaging, and MR angiography were not examined in this case. In the early stage of IVL, diffusion-weighted images might show a low apparent diffusion coefficient in an affected site, whereas perfusion MR imaging might reveal high rCBF and MR angiography might demonstrate segmental narrowing of the vessel, depending on the degree of infiltration of tumor.

The present case revealed very high rCBF on the first admission. After the first course of combination chemotherapy, the level of rCBF decreased to the normal range. Decreased rCBF of both cerebral hemispheres in brain SPECT was reported in a patient with IVL, accompanied by high-intensity lesions on T2-weighted in the brain and thoracic spinal cord. To our knowledge, there have been no reports describing high rCBF in IVL. IVL is known to mimic primary angiitis of the CNS (9), and angiography is nondiagnostic but suggest vasculitis (5). There were several reports that showed increased uptake of I-123 IMP or Tc-99m hexamethylpropyleneamine oxine (HMPAO) in patients with herpes simplex encephalitis, Japanese encephalitis, and viral encephalitis in acute to subacute phase (13–15). Also, it has been reported that early and delayed I-123 IMP uptake ratios comparing tumors with contralateral brain cortex (T/n ratio) of primary CNS lymphoma were significantly higher than those of nonlymphomatous tumors on both early and delayed images (16, 17). High rCBF observed in the present case may mean real high rCBF as the accumulation of lymphoma in itself like an inflammation process.

In the patient’s final clinical stage, 15 months after onset, she suffered from right hemiparesis for several days. Contrast-enhanced CT showed increased vascular attenuation in the entire left cerebral hemisphere. Walls of the vessels or perivascular space were enhanced by contrast material or by the slow flow of contrast material, because proliferation of tumor cells in the lumen of the vessels may have caused increased vascular attenuation. IMP-SPECT revealed high rCBF in the left cerebral hemisphere and the right cerebellum immediately after the symptom had abated. Increased I-123 IMP activity that was compatible with reperfusion hypereamia in a patient who suffered a subacute stroke and increased Tc-99m HMPAO in a cardioembolic mechanism of stroke have been reported (18, 19). It has been suggested that tumor cell infiltration to the left cerebral parenchyma induced the right hemiparesis and increased I-123 IMP uptake in the left cerebral hemisphere. Also reperfusion hypereamia was assumed after transit vascular occlusion because of tumor cells or dead tumor cells after chemotherapy. We could not, however, explain the reason why the half of the brain was affected.

Conclusion

As the disease progresses, MR imaging may reveal high-signal-intensity lesions on T2-weighted images. Elevated LDH or sIL-2R levels can be a good indicator of IVL; however, diagnosis of IVL remains difficult, especially at the early stage. Although the exact reason for high rCBF in IVL is still unknown, increased rCBF in SPECT may be helpful to diagnose early-stage IVL, in addition to other clinical findings, before cranial MR images can present nonspecific hyperintense lesions. In this case, effective chemotherapy could not suppress the progression of dementia, and autopsy revealed aggressive infiltration of tumor cells into the brain parenchyma. To improve the prognosis of the IVL, an accurate and early diagnosis is needed as soon as possible.

References

↵
Iida H, Itoh H, Nakazawa M, et al. Quantitative mapping of regional cerebral blood flow using iodine-123-IMP and SPECT. J Nucl Med 1994;35:2019–2030
Abstract/FREE Full Text
↵
Sheibani K, Battifora H, Winberg CD, et al. Further evidence that “malignant angioendotheliomatosis” is a angiotropic large-cell lymphoma. N Engl J Med 1986;314:943–948
PubMed
↵
Glass J, Hochberg FH, Miller DC. Intravascular lymphomatosis: a systemic disease with neurologic manifestations. Cancer 1993;71:3156–3164
CrossRef PubMed
↵
Pfleger L, Tappeiner J. Zur Kenntnis des systemisierten Endotheliomatose der cutanen Blutgafaesse. Hautarzt 1959;10:359–363
PubMed
↵
Calamia KT, Miller A, Shuster EA, et al. Intravascular lymphomatosis: a report of ten patients with central nervous system involvement and a review of the disease process. Adv Exp Med Biol 1999;455:249–265
PubMed
Chapin JE, Davis LE, Kornfeld M. Mandler RN. Neurologic manifestations of intravascular lymphomatosis. Acta Neurol Scand 1995;91:494–499
PubMed
↵
Lapkuviene O, Forchetti D, Roepke JE. Unusual sites of involvement by hematologic malignancies. Case 1. Intravascular large B-cell lymphoma presenting with CNS symptoms. J Clin Oncol 2001;19:3988–3991
FREE Full Text
↵
Mann RB. Are there site-specific differences among extranodal aggressive B-cell neoplasm? Am J Clin Pathol 1999;111(Suppl 1):S144–S150
↵
Beristain X, Azzarelli B. The neurological masquerade of intravascular lymphomatosis. Arch Neurol 2002;59:439–443
CrossRef PubMed
↵
Song DK, Boulis NM, McKeever PE, Quint DJ. Angiotropic large cell lymphoma with imaging characteristics of CNS vasculitis. AJNR Am J Neuroradiol 2002;23:239–242
Abstract/FREE Full Text
↵
Williams RL, Meltzer CC, Smirniotopoulos JG, et al. Cerebral MR imaging in intravascular lymphomaatosis. AJNR Am J Neuroradiol 1998;19:427–431
Abstract
↵
Terae S, Kudoh K, Ogata A, et al. MR appearance of the brain in intravascular lymphomatosis. Paper presented at the 17th neuroradiology symposium of the French Society of Neuroradiology, Paris, August2002
↵
Ito K, Sone S, Kondo Y, et al. N-isopropyl I-123 P-iodoamphetamine single photon emission computed tomography of herpes simplex encephalitis. Nippon Igaku Hoshasen Gakkai Zasshi 1989;49:1290–1297
PubMed
Kao CH, Wang SJ, Mak SC, et al. Viral encephalitis in children:detection with technetium-99m HMPAO brain single-photon emission CT and its value in prediction of outcome. AJNR Am J Neuroradiol 1994;15:1369–1373
Abstract/FREE Full Text
↵
Kimura K, Dosaka A, Hashimoto Y, et al. Single-photon emission CT findings in acute Japanese encephalitis. AJNR Am J Neuroradiol 1997;18:465–469
Abstract
↵
Yoshikai T, Fukahori T, Ishimaru J, et al. I-123 IMP SPET in the diagnosis of the primary central nervous system lymphoma. Eur J Nucl Med 2001;28:25–32
PubMed
↵
Akiyama Y, Moritake K, Yamasaki T, et al. The diagnostic value of 123I-IMP SPECT in non-Hodgkin’s lymphoma of the central nervous system. J Nucl Med 2000;41:1777–1783
Abstract/FREE Full Text
↵
Tamgac F, Moretti JL, Defer G, et al. Non-matched images with 123I-IMP and 99mTc-bicisate single-photon emission tomography in the demonstration of focal hyperaemia during the subacute phase of an ischemic stroke. Eur J Nucl Med 1994;21:254–257
PubMed
↵
Alexandrov AV, Ehrlich LE, Bladin CF, Black SE. Clinical significance of increased uptake of HMPAO on brain SPECT scans in acute stroke. J Neuroimaging 1996;6:150–155
PubMed

Received December 19, 2002.
Accepted after revision July 11, 2003.

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[1] ↵
Iida H, Itoh H, Nakazawa M, et al. Quantitative mapping of regional cerebral blood flow using iodine-123-IMP and SPECT. J Nucl Med 1994;35:2019–2030
Abstract/FREE Full Text

[2] ↵
Sheibani K, Battifora H, Winberg CD, et al. Further evidence that “malignant angioendotheliomatosis” is a angiotropic large-cell lymphoma. N Engl J Med 1986;314:943–948
PubMed

[3] ↵
Glass J, Hochberg FH, Miller DC. Intravascular lymphomatosis: a systemic disease with neurologic manifestations. Cancer 1993;71:3156–3164
CrossRef PubMed

[4] ↵
Pfleger L, Tappeiner J. Zur Kenntnis des systemisierten Endotheliomatose der cutanen Blutgafaesse. Hautarzt 1959;10:359–363
PubMed

[5] ↵
Calamia KT, Miller A, Shuster EA, et al. Intravascular lymphomatosis: a report of ten patients with central nervous system involvement and a review of the disease process. Adv Exp Med Biol 1999;455:249–265
PubMed

[6] Chapin JE, Davis LE, Kornfeld M. Mandler RN. Neurologic manifestations of intravascular lymphomatosis. Acta Neurol Scand 1995;91:494–499
PubMed

[7] ↵
Lapkuviene O, Forchetti D, Roepke JE. Unusual sites of involvement by hematologic malignancies. Case 1. Intravascular large B-cell lymphoma presenting with CNS symptoms. J Clin Oncol 2001;19:3988–3991
FREE Full Text

[8] ↵
Mann RB. Are there site-specific differences among extranodal aggressive B-cell neoplasm? Am J Clin Pathol 1999;111(Suppl 1):S144–S150

[9] ↵
Beristain X, Azzarelli B. The neurological masquerade of intravascular lymphomatosis. Arch Neurol 2002;59:439–443
CrossRef PubMed

[10] ↵
Song DK, Boulis NM, McKeever PE, Quint DJ. Angiotropic large cell lymphoma with imaging characteristics of CNS vasculitis. AJNR Am J Neuroradiol 2002;23:239–242
Abstract/FREE Full Text

[11] ↵
Williams RL, Meltzer CC, Smirniotopoulos JG, et al. Cerebral MR imaging in intravascular lymphomaatosis. AJNR Am J Neuroradiol 1998;19:427–431
Abstract

[12] ↵
Terae S, Kudoh K, Ogata A, et al. MR appearance of the brain in intravascular lymphomatosis. Paper presented at the 17th neuroradiology symposium of the French Society of Neuroradiology, Paris, August2002

[13] ↵
Ito K, Sone S, Kondo Y, et al. N-isopropyl I-123 P-iodoamphetamine single photon emission computed tomography of herpes simplex encephalitis. Nippon Igaku Hoshasen Gakkai Zasshi 1989;49:1290–1297
PubMed

[14] Kao CH, Wang SJ, Mak SC, et al. Viral encephalitis in children:detection with technetium-99m HMPAO brain single-photon emission CT and its value in prediction of outcome. AJNR Am J Neuroradiol 1994;15:1369–1373
Abstract/FREE Full Text

[15] ↵
Kimura K, Dosaka A, Hashimoto Y, et al. Single-photon emission CT findings in acute Japanese encephalitis. AJNR Am J Neuroradiol 1997;18:465–469
Abstract

[16] ↵
Yoshikai T, Fukahori T, Ishimaru J, et al. I-123 IMP SPET in the diagnosis of the primary central nervous system lymphoma. Eur J Nucl Med 2001;28:25–32
PubMed

[17] ↵
Akiyama Y, Moritake K, Yamasaki T, et al. The diagnostic value of 123I-IMP SPECT in non-Hodgkin’s lymphoma of the central nervous system. J Nucl Med 2000;41:1777–1783
Abstract/FREE Full Text

[18] ↵
Tamgac F, Moretti JL, Defer G, et al. Non-matched images with 123I-IMP and 99mTc-bicisate single-photon emission tomography in the demonstration of focal hyperaemia during the subacute phase of an ischemic stroke. Eur J Nucl Med 1994;21:254–257
PubMed

[19] ↵
Alexandrov AV, Ehrlich LE, Bladin CF, Black SE. Clinical significance of increased uptake of HMPAO on brain SPECT scans in acute stroke. J Neuroimaging 1996;6:150–155
PubMed

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A Case of Intravascular Lymphoma with Increased Regional Cerebral Blood Flow in I-123 IMP Single-Photon Emission CT

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