1naresh2naresh<pre>Array
(
    [urn:ac.highwire.org:guest:identity] => Array
        (
            [runtime-id] => urn:ac.highwire.org:guest:identity
            [type] => guest
            [service-id] => ajnr-ac.highwire.org
            [access-type] => Controlled
            [privilege] => Array
                (
                    [urn:ac.highwire.org:guest:privilege] => Array
                        (
                            [runtime-id] => urn:ac.highwire.org:guest:privilege
                            [type] => privilege-set
                            [privilege-set] => GUEST
                        )

                )

            [credentials] => Array
                (
                    [method] => guest
                )

        )

    [090b71eb-4470-46ea-ac6f-f05f1cbd0b54] => Array
        (
            [runtime-id] => 090b71eb-4470-46ea-ac6f-f05f1cbd0b54
            [type] => toll-free-key
            [service-id] => ajnr-ac.highwire.org
            [access-type] => Controlled
            [privilege] => Array
                (
                    [84eeffdb-ebad-421a-863f-95c728552783] => Array
                        (
                            [runtime-id] => 84eeffdb-ebad-421a-863f-95c728552783
                            [type] => toll-free-key
                        )

                )

            [credentials] => Array
                (
                    [method] => toll-free-key
                    [value] => tf_ipsecsha;eccb35e721dadd193a85711ba0636f90a6258dcc
                )

        )

)
1naresh2naresh<pre>Array
(
    [urn:ac.highwire.org:guest:identity] => Array
        (
            [runtime-id] => urn:ac.highwire.org:guest:identity
            [type] => guest
            [service-id] => ajnr-ac.highwire.org
            [access-type] => OpenAccess
            [privilege] => Array
                (
                    [urn:ac.highwire.org:guest:privilege] => Array
                        (
                            [runtime-id] => urn:ac.highwire.org:guest:privilege
                            [type] => privilege-set
                            [privilege-set] => GUEST
                        )

                )

            [credentials] => Array
                (
                    [method] => guest
                )

        )

    [befc8f6c-198c-4d7c-8c92-20432d768229] => Array
        (
            [runtime-id] => befc8f6c-198c-4d7c-8c92-20432d768229
            [type] => toll-free-key
            [service-id] => ajnr-ac.highwire.org
            [access-type] => OpenAccess
            [privilege] => Array
                (
                    [f1604ac0-f477-4263-9155-202487772b2f] => Array
                        (
                            [runtime-id] => f1604ac0-f477-4263-9155-202487772b2f
                            [type] => toll-free-key
                        )

                )

            [credentials] => Array
                (
                    [method] => toll-free-key
                    [value] => tf_ipsecsha;eccb35e721dadd193a85711ba0636f90a6258dcc
                )

        )

)
PT  - JOURNAL ARTICLE
AU  - El Khoury, R.
AU  - Misra, V.
AU  - Sharma, S.
AU  - Cox, C.S.
AU  - Walker, P.
AU  - Grotta, J.C.
AU  - Gee, A.
AU  - Suzuki, S.
AU  - Savitz, S.I.
TI  - The Effect of Transcatheter Injections on Cell Viability and Cytokine Release of Mononuclear Cells
AID  - 10.3174/ajnr.A2092
DP  - 2010 Sep 01
TA  - American Journal of Neuroradiology
PG  - 1488--1492
VI  - 31
IP  - 8
4099  - http://www.ajnr.org/content/31/8/1488.short
4100  - http://www.ajnr.org/content/31/8/1488.full
SO  - Am. J. Neuroradiol.2010 Sep 01; 31
AB  - BACKGROUND AND PURPOSE: Several studies suggest that various types of cellular therapies enhance recovery after stroke in animal models. IA-based delivery of cells to the brain is under investigation for stroke, but it is unknown whether cells are injured as a result of being injected through a catheter or exposed to iodinated contrast medium or solutions containing heparin. MATERIALS AND METHODS: We assessed the effect of catheterization with the Excelsior SL-10 catheter or exposure to heparin or iodine contrast on human bone marrow MNCs. Viability and cell injury were assessed by trypan blue exclusion, caspase-3 activity, and lipid peroxidation. Cellular function of MNCs was assessed by their production and release of VEGF, IL-10, and IGF-1. RESULTS: Flow rates of 10 million cells from 0.5 to 2 mL/min did not alter MNC viability; however, 5 mL/min of MNCs did reduce viability by 19%. Iodine and low-dose heparin exposure did not affect cell viability; however, high-dose heparin was cytotoxic. Catheter delivery at 2 mL/min did not affect levels of VEGF, IL-10, or IGF-1. CONCLUSIONS: MNCs do not appear to be damaged by heparin, iodine contrast, and the Excelsior SL-10 catheter at flow rates up to 2 mL/min. However, higher flow rates did reduce viability, and high-dose heparin did cause cell death. Cath-HypohypoxiaDMEMDulbecco&#039;s Modified Eagle&#039;s MediumELISAenzyme-linked immunosorbent assayFDAUS Food and Drug AdministrationH.D.high doseH-HypoxMNCs not catheterized but conditionedIAintra-arterialIGF-1insulin-like growth factor 1IL-10interleukin 10L.D.low doseMNCmononuclear cellN-Cathnonconditioned MNCsN-ContMNCs not catheterizedO.D.optical densityPBSphosphate-buffered salineVEGFvascular endothelial growth factor