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Experimental protocol*
Animal #μcathImaging ModalityFigure
11Ex vivo MPRAGE4
2–41In vivo DSC-MR5
5–72In vivo DSC-MR6
82In vivo microPET/T1 MR7
9–121In vivo laser Doppler−
13–162In vivo laser Doppler−
  • Note:—MPRAGE indicates magnetization-prepared rapid acquisition of gradient echo MR imaging; DSC-MR, dynamic susceptibility contrast-enhanced MR imaging.
  • * In animals #1 to #4 and #9 to #12, μcath1 was placed with its tip 22 to 23 mm distal to the common carotid bifurcation. In animals #5 to #8 and #13 to #16, μcath2 was placed with its tip 18 to 19 mm distal to the common carotid. Gd-DTPA was injected before (#1) or during (#2–#7) image acquisition. In animal #8, 18FDG was injected 5 minutes before imaging was performed. In animals #9 to #16, a μcath was left in place for up to 25 minutes, during which time NS was injected at a rate of 20 μL/min (#9–12) or 10 μL/min (#13–16). Data from animals #9 to #16 are described in the Results section but are not depicted graphically.