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Features of molecular imaging techniques used in reporter gene imaging
Imaging Technique Advantages Disadvantages
Bioluminescence optical imaging Very high sensitivity Very low spatial resolution
High throughput Only planar imaging, not tomographic
Very versatile Surface-weighted images owing to light scatter and absorption
Cheap Semiquantitative imaging data
Mass amount of probe required (? toxic)
Not an established clinical technique
MR imaging Very high spatial resolution Low sensitivity
Tomographic imaging Mass amount of probe required (? toxic)
Widely available established clinical technique 
PET, SPECT High sensitivity Low spatial resolution
Fully quantitative imaging data Probes for using HSV1-tk gene do not cross the blood-brain barrier
Tomographic imaging
Nanogram amount of probe required (nontoxic and safe) D2R gene normally expressed in basal ganglia interferes with image interpretation when using this reporter system
Established clinical techniques
Note:—? toxic indicates a question concerning potential toxicity.